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biotinylated horse antigoat secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated horse antigoat secondary antibody
    Biotinylated Horse Antigoat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+horse+antigoat+secondary+antibody/Biotinylated+Horse+Anti-Goat+IgG+Antibody/pmc12856726-92-26-32
    Average 96 stars, based on 1679 article reviews
    biotinylated horse antigoat secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Adolescent Vitamin D Supplementation Reverses Neuroplasticity and Motivational Deficits Induced by Developmental Alcohol Exposure and Early-Life Stress.
    Article Snippet: .. Sections were then incubated with a goat polyclonal anti- DCX antibody (1:400; Santa Cruz Biotechnology) at 4°C for 48 h. After rinsing, they were incubated with a biotinylated horse antigoat secondary antibody (1:200; Vector Laboratories) for 2 h. Immunoreactivity was visualized using an avidin- biotin– peroxidase complex (ABC Kit, Vector) and developed with DAB (Sigma- Aldrich) for approximately 3 min. .. Sections were mounted on gelatin- coated slides, dehydrated, cleared in xylene, and coverslipped with Entellan (Merck, USA) for subsequent analysis.

    Article Title: Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke
    Article Snippet: Sections were then incubated overnight at 4°C with goat anti-OX6 (MHC or MHCII; 1:750 BD), antibody markers in PBS supplemented with 3% normal serum and 0.1% triton X-100. .. Sections were then washed 3× with PBS and incubated in biotinylated horse antigoat secondary antibody (1:200; Vector Laboratories, Burlingame, CA) in PBS supplemented with normal horse serum, and 0.1% Triton X-100 for 1 hour. .. Next, the sections were incubated for 60 minutes in avidin–biotin substrate (ABC kit, Vector Laboratories, Burlingame, CA) and washed 3× with PBS for 10 minute each wash. All sections were then incubated for 1 minute in 3, 30-diaminobenzidine (DAB) with metal enhancer (Vector Laboratories) and washed 3× with PBS for 10 minutes each wash.

    Article Title: Adolescent Vitamin D Supplementation Reverses Neuroplasticity and Motivational Deficits Induced by Developmental Alcohol Exposure and Early‐Life Stress
    Article Snippet: .. Sections were then incubated with a goat polyclonal anti‐DCX antibody (1:400; Santa Cruz Biotechnology) at 4°C for 48 h. After rinsing, they were incubated with a biotinylated horse antigoat secondary antibody (1:200; Vector Laboratories) for 2 h. Immunoreactivity was visualized using an avidin‐biotin–peroxidase complex (ABC Kit, Vector) and developed with DAB (Sigma‐Aldrich) for approximately 3 min. .. Sections were mounted on gelatin‐coated slides, dehydrated, cleared in xylene, and coverslipped with Entellan (Merck, USA) for subsequent analysis.

    Article Title: Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke
    Article Snippet: Sections were then incubated overnight at 4°C with goat anti-OX6 (MHC or MHCII; 1:750 BD), antibody markers in PBS supplemented with 3% normal serum and 0.1% triton X-100. .. Sections were then washed 3× with PBS and incubated in biotinylated horse antigoat secondary antibody (1:200; Vector Laboratories, Burlingame, CA) in PBS supplemented with normal horse serum, and 0.1% Triton X-100 for 1 hour. .. Next, the sections were incubated for 60 minutes in avidin–biotin substrate (ABC kit, Vector Laboratories, Burlingame, CA) and washed 3× with PBS for 10 minute each wash. All sections were then incubated for 1 minute in 3, 30-diaminobenzidine (DAB) with metal enhancer (Vector Laboratories) and washed 3× with PBS for 10 minutes each wash.

    Avidin-Biotin Assay:

    Article Title: Adolescent Vitamin D Supplementation Reverses Neuroplasticity and Motivational Deficits Induced by Developmental Alcohol Exposure and Early-Life Stress.
    Article Snippet: .. Sections were then incubated with a goat polyclonal anti- DCX antibody (1:400; Santa Cruz Biotechnology) at 4°C for 48 h. After rinsing, they were incubated with a biotinylated horse antigoat secondary antibody (1:200; Vector Laboratories) for 2 h. Immunoreactivity was visualized using an avidin- biotin– peroxidase complex (ABC Kit, Vector) and developed with DAB (Sigma- Aldrich) for approximately 3 min. .. Sections were mounted on gelatin- coated slides, dehydrated, cleared in xylene, and coverslipped with Entellan (Merck, USA) for subsequent analysis.

    Article Title: Adolescent Vitamin D Supplementation Reverses Neuroplasticity and Motivational Deficits Induced by Developmental Alcohol Exposure and Early‐Life Stress
    Article Snippet: .. Sections were then incubated with a goat polyclonal anti‐DCX antibody (1:400; Santa Cruz Biotechnology) at 4°C for 48 h. After rinsing, they were incubated with a biotinylated horse antigoat secondary antibody (1:200; Vector Laboratories) for 2 h. Immunoreactivity was visualized using an avidin‐biotin–peroxidase complex (ABC Kit, Vector) and developed with DAB (Sigma‐Aldrich) for approximately 3 min. .. Sections were mounted on gelatin‐coated slides, dehydrated, cleared in xylene, and coverslipped with Entellan (Merck, USA) for subsequent analysis.

    Blocking Assay:

    Article Title: Idiopathic infertility in women is associated with distinct changes in proliferative phase uterine fluid proteins.
    Article Snippet: .. The sections were incubated for 30 minutes at RT with biotinylated horse antimouse secondary antibody (Vector Labs, BA-2000), biotinylated horse antigoat secondary antibody (Vector Labs, BA-9500) or biotinylated goat antirabbit secondary antibody (Vector Labs, BA-1000) at a dilution of 1:200v:v in non-immune blocking solution. .. Avidin-biotin conjugated with horseradish peroxidase (Vector Laboratories) at a dilution of 1:4000v:v in TBS was applied to each section and incubated for 30 minutes at RT.

    other:

    Article Title: Prostacyclin receptor up-regulates the expression of angiogenic genes in human endometrium via cross talk with epidermal growth factor Receptor and the extracellular signaling receptor kinase 1/2 pathway.
    Article Snippet: Nonspecific binding was further reduced by 20-min incubation with nonimmune horse serum (Vector Laboratories, Inc.) in a humidified chamber at room temperature before overnight incubation with the primary antibody at 4 C. For localization of IP receptor, slides were incubated with a goat polyclonal antibody raised against a peptide mapping near the carboxyl terminus of IP receptor of human origin (sc-20436, Santa Cruz Biotechnology, Inc.) at a 1:30 dilution in normal horse serum.



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